Attachment of staphylococci to various synthetic polymers

Abstract

Attachment of staphylococci to different synthetic polymers used for medical purposes was studied in applying the bioluminescent technique. The number of attached bacterial cells was determined by measuring the light emission resulting from the reaction between firefly luciferase and ATP present in adhered staphylococcal cells. It was shown that staphylococci attach to synthetic polymers within a few minutes, although one hour incubation is required to reach a constant maximum value of attached cells. Ten different synthetic polymers and five Staphyloccocus epidermidis strains were investigated in our study. The relationship between surface properties of polymers and bacterial attachment was studied. Various physicochemical parameters of synthetic polymers and bacteria were determined (contact angle, surface tension). It was demonstrated that bacterial attachment decreases with decreasing contact angle and with increasing surface tension of synthetic materials. Modifications of surface charge and hydrophobicity of solid materials were also investigated. It could be proved that especially negatively charged and hydrophilic synthetic polymers show very decreased staphylococcal attachment.