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Clinical Trial
. 1982;52(1):91-6.
doi: 10.1016/0022-1759(82)90353-2.

Alternative fluorochromes to ethidium bromide for automated read out of cytotoxicity tests

H J Tanke, P W van der Linden, J Langerak
Clinical Trial

Alternative fluorochromes to ethidium bromide for automated read out of cytotoxicity tests

H J Tanke et al. J Immunol Methods. 1982.

Abstract

Propidium iodide (PI) and diamidinophenylindol (DAPI) were compared with ethidium bromide (EB) in cytotoxicity tests. Double blind studies showed similar results with the 3 dyes in terms of percentages of dead (fluorescing) cells. The dyes were also investigated for their potential combination with the immunofluorescent membrane markers FITC and TRITC. Both EB-FITC and PI-FITC are suitable for conventional HLA-DR typing procedures. Because of its spectral properties, the DAPI-TRITC combination is promising for automated read out of DR-typing tests by image analysis systems.

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