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. 1982;13(1):48-52.
doi: 10.1007/BF00200200.

Production of a tumor-specific xenoantiserum from partially purified immunoprotective tumor antigen

T Tanaka, H Yamagishi, N R Pellis, B D Kahan

Production of a tumor-specific xenoantiserum from partially purified immunoprotective tumor antigen

T Tanaka et al. Cancer Immunol Immunother. 1982.

Abstract

Rabbits immunized with the immunoprotective TSTA fraction partially purified by preparative isoelectric focusing of 3 M KCl extracts from a chemically induced murine sarcoma, MCA-F, produced specific xenoantisera as assessed by an indirect membrane immunofluorescence assay. Only the immunizing tumor, MCA-F, and not the antigenically distinct MCA-D or MCA-T target cells were stained by the xenoantiserum. Absorption of anti-MCA-F antiserum with the antigenically distinct MCA-D or MCA-T cells did not reduce its capacity to bind to MCA-F cells. The immunofluorescence reaction was competitively inhibited by MCA-F fractions that induced specific immunoprotection: crude 3 M KCl extract, isoelectrically focused TSTA (fraction 15), and intact irradiated MCA-F cells. The TSTA specificity of these xenoantisera suggests that they may provide useful reagents for rapid isolation and characterization of the immunoprotective moiety.

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