doi: 10.1016/0378-1119(82)90212-8.
A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant
- PMID: 6762322
- DOI: 10.1016/0378-1119(82)90212-8
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A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant
Gene.
1982 Dec.
Abstract
Dictyostelium discoideum DNA fragments have been inserted into the chimeric bacterium-yeast plasmid YEp13. Recombinant plasmids were used to transform yeast using a strain of Saccharomyces cerevisiae deficient in OMP decarboxylase activity. Several clones were selected for growth in uracil-free medium. One clone was further analysed and contains a plasmid with a segment of D. discoideum DNA which complements a yeast ura3 mutation.
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