Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Dec;20(3):433-39.
doi: 10.1016/0378-1119(82)90212-8.

A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant

E Boy-Marcotte, M Jacquet

A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant

E Boy-Marcotte et al. Gene. 1982 Dec.

Abstract

Dictyostelium discoideum DNA fragments have been inserted into the chimeric bacterium-yeast plasmid YEp13. Recombinant plasmids were used to transform yeast using a strain of Saccharomyces cerevisiae deficient in OMP decarboxylase activity. Several clones were selected for growth in uracil-free medium. One clone was further analysed and contains a plasmid with a segment of D. discoideum DNA which complements a yeast ura3 mutation.

PubMed Disclaimer

Publication types

LinkOut - more resources