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. 1982 Dec;2(4):331-9.
doi: 10.1111/j.1600-0676.1982.tb00831.x.

An improved method for HBcAg demonstration in paraffin-embedded liver tissue

A Trevisan, F Gudat, C Busachi, E Stöcklin, L Bianchi

An improved method for HBcAg demonstration in paraffin-embedded liver tissue

A Trevisan et al. Liver. 1982 Dec.

Abstract

Factors influencing the preservation of HBcAg in formalin-fixed, paraffin-embedded liver tissue have been analysed. Fixation time, choice of the embedding medium, deparaffinization and selection of anti-HBc proved to be critical variables. For optimal results, fixation in 4% buffered formalin for no longer than 24-48 h, the use of wax with a low melting point, a heating step prior to deparaffinization, and a search for anti-HBc specifically reactive for paraffinated HBcAg are recommended.

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