doi: 10.1016/0304-3940(80)90081-6.
Simultaneous localization of [3H]estradiol and neurophysin I or arginine vasopressin in hypothalamic neurons demonstrated by a combined technique of dry-mount autoradiography and immunohistochemistry
- PMID: 6763162
- DOI: 10.1016/0304-3940(80)90081-6
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Simultaneous localization of [3H]estradiol and neurophysin I or arginine vasopressin in hypothalamic neurons demonstrated by a combined technique of dry-mount autoradiography and immunohistochemistry
Neurosci Lett.
1980 Apr.
Abstract
A combined technique of dry-mount autoradiography and immunoperoxidase staining is described for the localization of steroid hormones and neuropeptides in the same brain section. With this procedure [3H]estradiol is localized in nuclei of neurophysin and arginine vasopressin producing cells of the mouse supraoptic and paraventricular nucleus. The results suggest a direct action of estradiol on neurohypophysial hormone producing cells. This immunoautoradiographic technique is suitable for the simultaneous localization of steroid hormones and neuropeptides in the brain.
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