Effect of riboflavin on monoamine oxidase activity in cultured neuroblastoma cells
- PMID: 6765737
- PMCID: PMC11572776
- DOI: 10.1007/BF00716270
Effect of riboflavin on monoamine oxidase activity in cultured neuroblastoma cells
Abstract
Monoamine oxidase (MAO) depends on a covalently attached FAD cofactor for activity. Activity is depressed in mouse neuroblastoma cells (N1E-115) grown in synthetic N2 medium lacking riboflavin. MAO activity in depleted cells is stimulated by added riboflavin, and this recovery is blocked by inhibitors of RNA and protein synthesis, and not by an inhibitor of protein glycosylation Recovery from riboflavin depletion appears to depend upon new RNA and protein synthesis, and not on the addition of FAD cofactor to an inactive MAO precursor.
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References
-
- Addison, R., and McCormick, D. B. (1978). Biogenesis of flavoprotein and cytochrome components in hepatic mitochondria from riboflavin-deficient rats.Biochem. Biophys. Res. Commun.81133–138. - PubMed
-
- Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem.72248–254. - PubMed
-
- Breakefield, X. O., Castiglione, C., Edelstein, S. (1976). Monoamine oxidase activity decreased in cells lacking hypoxanthine phosphoribosyltransferase activity.Science1921018–1020. - PubMed
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