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. 1981 Dec;1(4):351-9.
doi: 10.1007/BF00716270.

Effect of riboflavin on monoamine oxidase activity in cultured neuroblastoma cells

V Bonnefil  1 , C M Castiglione, R M Cawthon, X O Breakefield
Affiliations

Effect of riboflavin on monoamine oxidase activity in cultured neuroblastoma cells

V Bonnefil et al. Cell Mol Neurobiol. 1981 Dec.

Abstract

Monoamine oxidase (MAO) depends on a covalently attached FAD cofactor for activity. Activity is depressed in mouse neuroblastoma cells (N1E-115) grown in synthetic N2 medium lacking riboflavin. MAO activity in depleted cells is stimulated by added riboflavin, and this recovery is blocked by inhibitors of RNA and protein synthesis, and not by an inhibitor of protein glycosylation Recovery from riboflavin depletion appears to depend upon new RNA and protein synthesis, and not on the addition of FAD cofactor to an inactive MAO precursor.

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