doi: 10.1007/BF00716273.
Uptake and release of choline in cultures of human glioma cells
Affiliations
- PMID: 6765739
- PMCID: PMC11572855
- DOI: 10.1007/BF00716273
Item in Clipboard
Uptake and release of choline in cultures of human glioma cells
Cell Mol Neurobiol.
1981 Dec.
Abstract
Human glioma cells (138MG) have a low-affinity uptake system for choline (Km = 20 microM; Vmax = 56 pmol/min/10(6) cells). The uptake is reduced by acetylcholine, hemicholinium-3, HgCl2, and phosphodiesterase inhibitors. Release of [3H]choline from preloaded cultures showed two pools with half-lives of 1.3 and 160 min. Choline release was stimulated by 8-bromo-cAMP or isobutylmethylxanthine. The results suggest that release of choline occurs by a facilitated diffusion transport system and is increased by elevations of intracellular cAMP.
Similar articles
-
Hemicholinium-3 derivatives A-4 and A-5 affect choline and acetylcholine metabolism.Eur J Pharmacol. 1991 Feb 25;206(2):105-12. doi: 10.1016/0922-4106(91)90018-d. Eur J Pharmacol. 1991. PMID: 2065716
-
Pharmacological characteristics of choline transport system in mouse cerebral cortical neurons in primary culture.Jpn J Pharmacol. 1991 Feb;55(2):223-32. doi: 10.1254/jjp.55.223. Jpn J Pharmacol. 1991. PMID: 2067141
-
Choline uptake in cholinergic nodose cell bodies.Neuroscience. 1991;43(2-3):687-96. doi: 10.1016/0306-4522(91)90327-k. Neuroscience. 1991. PMID: 1922789
-
Cholinergic differentiation of clonal rat pheochromocytoma cells (PC12) induced by factors contained in glioma-conditioned medium: enhancement of high-affinity choline uptake system and reduction of norepinephrine uptake system.Brain Res. 1986 Jan;389(1-2):145-52. doi: 10.1016/0165-3806(86)90182-3. Brain Res. 1986. PMID: 3948005
-
Functional characterization of a high-affinity choline transport system in human keratinocytes.J Invest Dermatol. 2002 Jul;119(1):118-21. doi: 10.1046/j.1523-1747.2002.01801.x. J Invest Dermatol. 2002. PMID: 12164933
Cited by
-
Growth and morphology of neuronal cell lines cultured in perfusion.In Vitro. 1983 Dec;19(12):875-80. doi: 10.1007/BF02661707. In Vitro. 1983. PMID: 6363277
References
-
- Ansell, G. B., and Spanner, S. (1979). Sources of choline for acetylcholine synthesis in the brain. InNutrition and the Brain, Vol. 5 (A. Barbeau, J. H. Growdon, and R. J. Wurtman Eds.) Raven Press, New York.
-
- Arlock, P., and Kanje, M. (1977). Effects of dibuturyl cyclic AMP on the transmembrane potentials of cultured human glioma cells.Exp. Cell Res.109105–108. - PubMed
-
- Edström, A., Haglid, K. G., Kanje, M., Rönnbäck, L., and Walum, E. (1973). Morphological alterations and increase of S-100 protein in cultured human glioma cells deprived of serum.Exp. Cell Res.83426–429. - PubMed
-
- Edström, A., Kanje, M., and Walum, E. (1974). Effects of dibuturyl cyclic AMP and prostaglandin El on cultured human glioma cells.Exp. Cell Res.85271–223. - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources