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. 1981 Dec;1(4):389-99.
doi: 10.1007/BF00716273.

Uptake and release of choline in cultures of human glioma cells

E Walum  1
Affiliations

Uptake and release of choline in cultures of human glioma cells

E Walum. Cell Mol Neurobiol. 1981 Dec.

Abstract

Human glioma cells (138MG) have a low-affinity uptake system for choline (Km = 20 microM; Vmax = 56 pmol/min/10(6) cells). The uptake is reduced by acetylcholine, hemicholinium-3, HgCl2, and phosphodiesterase inhibitors. Release of [3H]choline from preloaded cultures showed two pools with half-lives of 1.3 and 160 min. Choline release was stimulated by 8-bromo-cAMP or isobutylmethylxanthine. The results suggest that release of choline occurs by a facilitated diffusion transport system and is increased by elevations of intracellular cAMP.

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References

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