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. 1980 Mar 15;36(3):347-9.
doi: 10.1007/BF01952319.

Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay

N Monji, A Castro

Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay

N Monji et al. Experientia. .

Abstract

A new cortisol derivative, cortisol-21-m-maleimidobenzoate (CMB), was synthesized and conjugated with sulfhydryl groups of beta-galactosidase (BG). Both CMB-BG and CHS-BG conjugates have a high immunoreactivity to cortisol antibody, and although CHS-BG does not displace well with the added cortisol, CMB-BG does.

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