Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay
- PMID: 6768582
- DOI: 10.1007/BF01952319
Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay
Abstract
A new cortisol derivative, cortisol-21-m-maleimidobenzoate (CMB), was synthesized and conjugated with sulfhydryl groups of beta-galactosidase (BG). Both CMB-BG and CHS-BG conjugates have a high immunoreactivity to cortisol antibody, and although CHS-BG does not displace well with the added cortisol, CMB-BG does.