doi: 10.1177/28.4.6768792.
Embedment in glycol methacrylate at low temperature allows immunofluorescent localization of a labile tissue protein
- PMID: 6768792
- DOI: 10.1177/28.4.6768792
Item in Clipboard
Embedment in glycol methacrylate at low temperature allows immunofluorescent localization of a labile tissue protein
J Histochem Cytochem.
1980 Apr.
Abstract
The antigenic activity of a labile protein postulated to the hormone ovine chorionic somatomammotropin (OcS) is preserved in tissue fixed in either glutaraldehyde or paraformaldehyde and low temperature-embedded in the water-soluble plastic glycol methacrylate. Immunofluorescence techniques used on 1--2 micrometer thick sections show that this protein is located in the cytoplasm of certain fetal chorionic cells in association with small spherical bodies, which may be lipid. The use of borohydrate reduction or treatment with Schiff's reagent to reduce glutaraldehyde-induced background fluorescence is described.
Similar articles
-
The cellular localization of chorionic somatomammotrophin in ovine chorion.J Reprod Fertil. 1982 Sep;66(1):9-16. doi: 10.1530/jrf.0.0660009. J Reprod Fertil. 1982. PMID: 6750111
-
Immunofluorescent localization of pea storage proteins in glycol methacrylate embedded tissue.J Histochem Cytochem. 1979 Oct;27(10):1312-6. doi: 10.1177/27.10.390032. J Histochem Cytochem. 1979. PMID: 390032
-
[Immunologic properties of ovine chorionic somatomammotropin].C R Acad Hebd Seances Acad Sci D. 1978 Jan 16;286(2):245-8. C R Acad Hebd Seances Acad Sci D. 1978. PMID: 417875 French.
-
Immunohistological staining of antigens on semithin sections of specimens embedded in plastic (GMA-Quetol 523).J Immunol Methods. 1979;30(3):277-88. doi: 10.1016/0022-1759(79)90102-9. J Immunol Methods. 1979. PMID: 115925
-
Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure.Immunol Rev. 1991 Feb;119:65-93. doi: 10.1111/j.1600-065x.1991.tb00578.x. Immunol Rev. 1991. PMID: 2045123 Review. No abstract available.
Cited by
-
Expression of a specific marker of avian programmed cell death in both apoptosis and necrosis.Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8641-5. doi: 10.1073/pnas.91.18.8641. Proc Natl Acad Sci U S A. 1994. PMID: 8078937 Free PMC article.
-
Immunofluorescent localization of two water-soluble glycoproteins including the major allergen from the pollen of ryegrass, Lolium perenne.Histochem J. 1981 May;13(3):461-80. doi: 10.1007/BF01005062. Histochem J. 1981. PMID: 7019166
-
A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry.Histochem J. 1985 Apr;17(4):467-75. doi: 10.1007/BF01003206. Histochem J. 1985. PMID: 4044281
-
Immunochemical localization of phenylalanine ammonia-lyase and chalcone synthase in anthers.Planta. 1985 Feb;163(2):183-90. doi: 10.1007/BF00393504. Planta. 1985. PMID: 24249336
-
Clathrin and clathrin-accessory proteins in rat kidney cortex epithelia.Histochem Cell Biol. 2006 Aug;126(2):219-29. doi: 10.1007/s00418-006-0169-2. Epub 2006 Apr 20. Histochem Cell Biol. 2006. PMID: 16625367
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
