doi: 10.1016/s0022-1759(80)80008-1.
An automated fluorimetric assay for the simultaneous determinations of histamine and 5-hydroxytryptamine released from rat mast cell preparations
- PMID: 6768805
- DOI: 10.1016/s0022-1759(80)80008-1
Item in Clipboard
An automated fluorimetric assay for the simultaneous determinations of histamine and 5-hydroxytryptamine released from rat mast cell preparations
J Immunol Methods.
1980.
Abstract
An automated assay suitable for the extraction and simultaneous fluorimetric determination of histamine and 5-hydroxytryptamine (5-HT) released from rat isolated peritoneal mast cells is described. The method gave good reproducibility and accuracy in use and required a sample volume of 0.5 ml for assay at a sampling rate of 30/h. The system was evaluated by assessment of its performance in the determination of histamine and 5-HT released from rat mast cells in response to compound 48/80. The detection limit for the procedure was 2 ng 5-HT base/ml and 20 ng histamine base/ml.
Similar articles
-
Compound 48/80 and substance P induced release of histamine and serotonin from rat peritoneal mast cells.Agents Actions. 1983 Apr;13(2-3):138-41. doi: 10.1007/BF01967317. Agents Actions. 1983. PMID: 6191543
-
The effect of adrenocorticotropin on histamine and 5-hydroxytryptamine secretion from rat mast cells.Agents Actions. 1984 Apr;14(3-4):454-7. doi: 10.1007/BF01973848. Agents Actions. 1984. PMID: 6203367
-
Comparison of histamine and 5-hydroxytryptamine content and secretion in rat mast cells isolated from different anatomical locations.Int Arch Allergy Appl Immunol. 1989;90(4):382-6. doi: 10.1159/000235058. Int Arch Allergy Appl Immunol. 1989. PMID: 2482258
-
Parallel secretion of endogenous 5-hydroxytryptamine and histamine from mast cells stimulated by vasoactive peptides and compound 48/80.Br J Pharmacol. 1984 Feb;81(2):227-9. doi: 10.1111/j.1476-5381.1984.tb10069.x. Br J Pharmacol. 1984. PMID: 6200170 Free PMC article.
-
The measurement of histamine release from suspensions of rat peritoneal cells rich in mast cells.Agents Actions. 1977 Oct;7(4):431-6. doi: 10.1007/BF01966848. Agents Actions. 1977. PMID: 73348
Cited by
-
Compound 48/80 and substance P induced release of histamine and serotonin from rat peritoneal mast cells.Agents Actions. 1983 Apr;13(2-3):138-41. doi: 10.1007/BF01967317. Agents Actions. 1983. PMID: 6191543
-
The effect of adrenocorticotropin on histamine and 5-hydroxytryptamine secretion from rat mast cells.Agents Actions. 1984 Apr;14(3-4):454-7. doi: 10.1007/BF01973848. Agents Actions. 1984. PMID: 6203367
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
