[Demonstration of the Bovid herpesvirus 2 in cell cultures with the aid of fluorescent antibodies]

Abstract

Used was the immunofluorescence method employing fluorescinisothiocyanate-labeled antibodies against Bovid herpesvirus 2. The localisation and the dynamics of the virus antigen were followed up in cell cultures of calf kidney epithelium. Results showed that there was a specific cytoplastic-nuclear fluorescence in the infected cells. The dynamics of the virus multiplication revealed the presence of a specific antigen by about the 6th hour following the infection of the cells. Discussed is the problem of using the immunofluorescence technique in the differentiation of herpes mammillitis from the rest vesicular diseases of the cow's udder that are similar to one another by clinical picture.