Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Jun;142(3):836-42.
doi: 10.1128/jb.142.3.836-842.1980.

Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase

D E Ohman, S J Cryz, B H Iglewski

Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase

D E Ohman et al. J Bacteriol. 1980 Jun.

Abstract

Pseudomonas aeruginosa PAO mutants defective in elastase were isolated by plate assays of nitrosoguanidine-mutagenized clones. A total of 75 elastase mutants were isolated from 43,000 mutagenized clones. One mutant (PAO-E64) was apparently identical to the parental strain except for its deficiency in elastase activity. This mutant produced an enzyme which was antigenically indistinguishable from parental elastase. Furthermore, equal levels of elastase antigen were produced by this mutant and its parental strain. The mutant elastase, however, had greatly reduced enzymatic activity. Mutant PAO-E64 is presumed to have a mutation in the structural gene for elastase. We have designated the genotype of the mutation in PAO-E64 as lasA1.

PubMed Disclaimer

References

    1. J Immunol. 1970 Jan;104(1):140-7 - PubMed
    1. J Clin Endocrinol Metab. 1972 Jan;34(1):23-8 - PubMed
    1. Genet Res. 1972 Feb;19(1):91-108 - PubMed
    1. Infect Immun. 1973 Feb;7(2):212-7 - PubMed
    1. J Infect Dis. 1974 Feb;129(2):117-23 - PubMed

Publication types