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. 1980;34(2):133-9.
doi: 10.1016/0022-1759(80)90167-2.

A simple method of labelling mouse Thy-1 antibodies with FITC

M M Gani, T Hunt, J M Summerell

A simple method of labelling mouse Thy-1 antibodies with FITC

M M Gani et al. J Immunol Methods. 1980.

Abstract

Unlike antibodies produced in rabbits and other animals, mouse antibodies have proved to be difficult to covalently label with fluorochromes. By careful control of the labelling conditions, a method has been established for labelling mouse Thy-1 antibodies obtained by immunisation with normal thymus cells. The resultant FITC Thy-1 antibodies suffer little loss in cytotoxic properties, react with the correct specificity and can be used to label Thy-1 positive cells.

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