Comparative Study
doi: 10.1007/BF00329546.
Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII
- PMID: 6771110
- DOI: 10.1007/BF00329546
Item in Clipboard
Comparative Study
Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII
Chromosoma.
1980.
Abstract
Macronuclear DNA s from three related hypotrichous ciliated protozoans were compared by agarose gel electrophoresis. Each was shown to be composed of DNA duplexes that yielded a unique pattern of bands overlying a continuous distribution of DNA sizes ranging from approximately 400 bp to approximately 20,000 bp. By EM, the number average molecular sizes for double-stranded DNA were 2,200 bp for Oxytricha sp., 2,514 bp for Stylonychia pustulata and 18,836 bp for Euplotes aediculatus. Contrary to previous reports we present evidence that the macronuclear DNA s in each of these three organisms lack single-stranded interruptions.
Similar articles
-
The organization of macronuclear rDNA molecules of four hypotrichous ciliated protozoans.Chromosoma. 1982;85(1):1-9. doi: 10.1007/BF00344590. Chromosoma. 1982. PMID: 6284451
-
Inverted repetitious sequences in the macronuclear DNA of hypotrichous ciliates.Proc Natl Acad Sci U S A. 1975 Feb;72(2):678-82. doi: 10.1073/pnas.72.2.678. Proc Natl Acad Sci U S A. 1975. PMID: 804696 Free PMC article.
-
Inverted terminal repeat sequence in the macronuclear DNA of Stylonychia pustulata.Gene. 1980 Sep;10(4):301-6. doi: 10.1016/0378-1119(80)90150-x. Gene. 1980. PMID: 6776007
-
The unusual way to make a genetically active nucleus.DNA Cell Biol. 2009 Feb;28(2):71-8. doi: 10.1089/dna.2008.0806. DNA Cell Biol. 2009. PMID: 19196049 Review.
-
Macronuclear gene-sized molecules of hypotrichs.Nucleic Acids Res. 1995 Apr 25;23(8):1279-83. doi: 10.1093/nar/23.8.1279. Nucleic Acids Res. 1995. PMID: 7753617 Free PMC article. Review.
Cited by
-
Developmentally regulated chromosome fragmentation linked to imprecise elimination of repeated sequences in paramecia.Eukaryot Cell. 2003 Oct;2(5):1076-90. doi: 10.1128/EC.2.5.1076-1090.2003. Eukaryot Cell. 2003. PMID: 14555491 Free PMC article.
-
Alternative use of chromosome fragmentation sites in the ciliated protozoan Oxytricha nova.Nucleic Acids Res. 1988 Jan 11;16(1):251-64. doi: 10.1093/nar/16.1.251. Nucleic Acids Res. 1988. PMID: 2829118 Free PMC article.
-
Reordering of nine exons is necessary to form a functional actin gene in Oxytricha nova.Proc Natl Acad Sci U S A. 1989 Aug;86(16):6264-8. doi: 10.1073/pnas.86.16.6264. Proc Natl Acad Sci U S A. 1989. PMID: 2503830 Free PMC article.
-
Sequencing of random Euplotes crassus macronuclear genes supports a high frequency of +1 translational frameshifting.Eukaryot Cell. 2005 Dec;4(12):2098-105. doi: 10.1128/EC.4.12.2098-2105.2005. Eukaryot Cell. 2005. PMID: 16339727 Free PMC article.
-
In vitro aggregation of the gene-sized DNA molecules of the ciliate Stylonychia mytilus.Proc Natl Acad Sci U S A. 1980 Jul;77(7):4104-7. doi: 10.1073/pnas.77.7.4104. Proc Natl Acad Sci U S A. 1980. PMID: 6776521 Free PMC article.