doi: 10.1007/BF00270475.
High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: construction and characterization of recombinant strains with suppressor-enhancing mutations
- PMID: 6771486
- DOI: 10.1007/BF00270475
Item in Clipboard
High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: construction and characterization of recombinant strains with suppressor-enhancing mutations
Mol Gen Genet.
1980.
Abstract
A set of eight strains combining the supD43,74 ts1 suppressor gene with alleles of three suppressor-enhancing (sue) genes have been constructed and characterized. The sue mutations work cooperatively to raise suppressor activity and together raise the activity of the supD43,74-encoded suppressor 40-fold. These strains further expand the utility of the ts suppressor system by providing as much as 100% suppressor activity at temperatures at or below 20 degrees C to as little as 0.015% suppressor activity at 43 degrees C.
Similar articles
-
High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: isolation of strains with suppressor-enhancing mutations.Mol Gen Genet. 1980;177(4):545-52. doi: 10.1007/BF00272662. Mol Gen Genet. 1980. PMID: 6991863
-
High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli.J Bacteriol. 1981 Jan;145(1):459-65. doi: 10.1128/jb.145.1.459-465.1981. J Bacteriol. 1981. PMID: 7007324 Free PMC article.
-
An amber mutation in the gene encoding the beta' subunit of Escherichia coli RNA polymerase.J Bacteriol. 1982 Nov;152(2):736-46. doi: 10.1128/jb.152.2.736-746.1982. J Bacteriol. 1982. PMID: 6290449 Free PMC article.
-
Effects of surrounding sequence on the suppression of nonsense codons.J Mol Biol. 1983 Feb 15;164(1):59-71. doi: 10.1016/0022-2836(83)90087-6. J Mol Biol. 1983. PMID: 6188840
-
Temperature-sensitive amber suppression of ompF'-'lacZ fused gene expression in a supE mutant of Escherichia coli K12.FEMS Microbiol Lett. 1993 Feb 15;107(1):71-6. doi: 10.1016/0378-1097(93)90356-7. FEMS Microbiol Lett. 1993. PMID: 8468002
Cited by
-
Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli.Microbiol Rev. 1988 Sep;52(3):354-74. doi: 10.1128/mr.52.3.354-374.1988. Microbiol Rev. 1988. PMID: 3054467 Free PMC article. Review. No abstract available.
-
Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli.J Bacteriol. 1988 Nov;170(11):5378-81. doi: 10.1128/jb.170.11.5378-5381.1988. J Bacteriol. 1988. PMID: 3053663 Free PMC article.
-
Rhizobium meliloti suhR suppresses the phenotype of an Escherichia coli RNA polymerase sigma 32 mutant.J Bacteriol. 1990 Jul;172(7):3559-68. doi: 10.1128/jb.172.7.3559-3568.1990. J Bacteriol. 1990. PMID: 2113906 Free PMC article.
-
Isolation, purification, and in vitro characterization of recessive-lethal-mutant RNA polymerases from Escherichia coli.J Bacteriol. 1996 Sep;178(17):5263-71. doi: 10.1128/jb.178.17.5263-5271.1996. J Bacteriol. 1996. PMID: 8752347 Free PMC article.
-
Colicin A receptor: role of two Escherichia coli outer membrane proteins (OmpF protein and btuB gene product) and lipopolysaccharide.J Bacteriol. 1982 Aug;151(2):983-8. doi: 10.1128/jb.151.2.983-988.1982. J Bacteriol. 1982. PMID: 7047502 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
