Isolation and mapping of a cloned ribosomal protein gene of Drosophila melanogaster

Abstract

Molecular cloning techniques are particularly well suited to the study of gene organization in Drasophila melanogaster because recombinant DNA can easily be localized in the genome by in situ hybridization to salivary gland polytene chromosomes. We report here the isolation and preliminary characterization of a recombinant phage, designated C25, containing a bona fide D. melanogaster ribosomal protein gene. In situ hybridization demonstrates that this sequence maps to region 99D on chromosome 3.