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. 1980 Aug;26(9):1255-60.

Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay

V C Tsang, B C Wilson, S E Maddison
  • PMID: 6772340

Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay

V C Tsang et al. Clin Chem. 1980 Aug.

Abstract

We describe a single-cuvette enzyme-linked immunosorbent assay (ELISA) based on enzymic rate kinetics. This kinetic assay can yield linear quantitative data on immunoglobin concentrations. Optimum assay conditions, component concentrations, reaction intervals, and pH are described. Assay linearity and sensitivity are demonstrated in systems involving immunoglobulin G and antibodies to it, and with pooled sera from monkeys infected with Schistosoma mansoni.

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