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. 1980 Jul;29(1):281-3.
doi: 10.1128/iai.29.1.281-283.1980.

Immunobead enzyme-linked immunosorbent assay for quantitating immunoglobulin A in human secretions and serum

D A Sack, P K Neogi, M K Alam

Immunobead enzyme-linked immunosorbent assay for quantitating immunoglobulin A in human secretions and serum

D A Sack et al. Infect Immun. 1980 Jul.

Abstract

We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of 10 ng/ml, and can be completed in 1 day. Since there is no diffusion through agar, the assay does not require a correction factor when measuring secretory immunoglobulin A. This method should be useful in studies of local mucosal immunity in humans, especially when a very sensitive assay is needed.

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