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Comparative Study
. 1978 Jun;35(6):1008-11.
doi: 10.1128/aem.35.6.1008-1011.1978.

Inclusion of xylan in a medium for the enumeration of total culturable rumen bacteria

P A Henning, A E van der Walt
Comparative Study

Inclusion of xylan in a medium for the enumeration of total culturable rumen bacteria

P A Henning et al. Appl Environ Microbiol. 1978 Jun.

Abstract

The influence of the inclusion of xylan in a medium for enumeration of total culturable rumen bacteria was investigated. Maximum colony numbers were obtained on a medium, GCSX-2, which contained 0.033% each glucose and cellobiose and 0.067% each soluble starch and xylan. This medium gave higher colony counts than either medium 98-5 of Bryant and Robinson (J. Dairy Sci. 44:1446-1456, 1961), medium 98-5 of Chung and Hungate (Appl. Environ. Microbiol. 32:649-652, 1976), containing an added lucerne (hemicellulose + cellulose) fiber substrate, or medium GCSX-2 with the added lucerne (hemicellulose + cellulose) fraction. The time of collection of rumen fluid influenced the colony counts on the media containing the lucerne fiber substrate but was without effect on medium GCSX-2.

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References

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    1. Appl Environ Microbiol. 1976 Nov;32(5):703-10 - PubMed
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