Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Oct 1;191(1):111-6.
doi: 10.1042/bj1910111.

Production of a variant of beta-lactamase II with selectively decreased cephalosporinase activity by a mutant of Bacillus cereus 569/H/9

G S Baldwin, G F Edwards, P A Kiener, M J Tully, S G Waley, E P Abraham

Production of a variant of beta-lactamase II with selectively decreased cephalosporinase activity by a mutant of Bacillus cereus 569/H/9

G S Baldwin et al. Biochem J. .

Abstract

1. Mutants of Bacillus cereus 569/H/9 have been screened in a search for strains that synthesize variants of beta-lactamase II. 2. One of these mutants (strain 569/H/9/1) produces a beta-lactamase II-like enzyme that shows a selective decrease in cephalosporinase activity. 3. beta-Lactamase II from strain 569/H/9/1 has been purified to apparent homogeneity and its kinetic properties have been examined. This enzyme resembles the parent beta-lactamase II in its relative activity with benzylpenicillin as substrate when Zn(II) is replaced by other metal ions, but differs detectably from the parent enzyme in its isoelectric point.

PubMed Disclaimer

References

    1. Biochem J. 1974 Oct;143(1):115-27 - PubMed
    1. Biochem J. 1974 Oct;143(1):129-35 - PubMed
    1. J Gen Microbiol. 1954 Dec;11(3):493-505 - PubMed
    1. Biochem J. 1966 Jan;98(1):1-16P - PubMed
    1. Biochem J. 1963 Sep;88:452-9 - PubMed

Publication types