[Analysis of the stability and local cooperativity of DNA: proposal of a molecular mechanism for the initiation of the transcription of gene A3 of bacteriophage T7 by the RNA polymerase from E. coli]
[Article in
French]
- PMID: 6783340
Item in Clipboard
[Analysis of the stability and local cooperativity of DNA: proposal of a molecular mechanism for the initiation of the transcription of gene A3 of bacteriophage T7 by the RNA polymerase from E. coli]
[Article in
French]
C R Seances Acad Sci III.
.
Abstract
RNA polymerase (RNAP) complexed to the A3 promoter of bacteriophage T7 is known to unwind a DNA segment located downstream of the Pribnow box. This finding can be accounted for if it is assumed that the subunit sigma of RNAP unstabilizes three GC base pairs located just upstream of the transcription start. As a consequence, the rate of promoter utilisation might be related to the relative stability of the DNA between the "Pribnow box" and the transcription start.
Similar articles
-
[Properties of cloned promoters].Mol Biol (Mosk). 1984 Jan-Feb;18(1):130-9. Mol Biol (Mosk). 1984. PMID: 6200764 Russian.
-
[Analysis of the stability and local cooperativity of DNA : elements permitting the recognition of promoters by DNA-dependent RNA polymerase].C R Seances Acad Sci III. 1981 Jan 5;292(1):5-8. C R Seances Acad Sci III. 1981. PMID: 6786772 French.
-
Promoter recognition and transcription initiation in E. coli.Folia Biol (Praha). 1984;30 Spec No:105-18. Folia Biol (Praha). 1984. PMID: 6373393
-
E. coli RNA polymerase interacts homologously with two different promoters.Cell. 1980 Jun;20(2):269-81. doi: 10.1016/0092-8674(80)90613-3. Cell. 1980. PMID: 6248238 Review.
-
[Interaction of bacterial RNA polymerase with the promotor region].Postepy Biochem. 1982;28(4):465-86. Postepy Biochem. 1982. PMID: 6764808 Review. Polish. No abstract available.
Cited by
-
Physical characteristics in eucaryotic promoters.Nucleic Acids Res. 1983 Jul 11;11(13):4521-40. doi: 10.1093/nar/11.13.4521. Nucleic Acids Res. 1983. PMID: 6306592 Free PMC article.
-
Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: evolutionary relationships and transcriptional analysis of aphA-3 genes.Mol Gen Genet. 1987 May;207(2-3):509-13. doi: 10.1007/BF00331623. Mol Gen Genet. 1987. PMID: 3039302
-
DNA sequences specifying the transcription of the streptococcal kanamycin resistance gene in Escherichia coli and Bacillus subtilis.Mol Gen Genet. 1985;198(2):348-52. doi: 10.1007/BF00383017. Mol Gen Genet. 1985. PMID: 3920478
MeSH terms
Substances
LinkOut - more resources
Research Materials
Miscellaneous