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. 1980 Aug;29(2):831-4.
doi: 10.1128/iai.29.2.831-834.1980.

Radioiodination of an outer membrane protein in intact Rickettsia prowazekii

D K Smith, H H Winkler

Radioiodination of an outer membrane protein in intact Rickettsia prowazekii

D K Smith et al. Infect Immun. 1980 Aug.

Abstract

Intact Rickettsia prowazekii was radiolabeled with the glucose oxidase-lactoperoxidase method of iodination. Separation of the rickettsial extract into cytoplasmic, outer and inner membrane fractions demonstrated that the outer membrane was preferentially labeled. Analysis of the polypeptides of these fractions on high-resolution slab polyacrylamide gels showed that most of the 125I was in polypeptide T49, an outer membrane constituent. Additional outer membrane polypeptides were iodinated in broken envelope preparations, demonstrating that T49 is uniquely accessible to the external environment and the asymmetric polypeptide organization of the outer membrane.

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