Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Mar;78(3):1643-6.
doi: 10.1073/pnas.78.3.1643.

Resonance Raman spectroscopy of specifically [epsilon-15N]lysine-labeled bacteriorhodopsin

P V Argade, K J Rothschild, A H Kawamoto, J Herzfeld, W C Herlihy

Resonance Raman spectroscopy of specifically [epsilon-15N]lysine-labeled bacteriorhodopsin

P V Argade et al. Proc Natl Acad Sci U S A. 1981 Mar.

Abstract

The possible interaction of a second lysine with the retinylidene Schiff base of bacteriorhodopsin (Lewis, A., Marcus, M. A., Ehrenberg, B. & Crespi, H. (1978) Proc. Natl. Acad. Sci. USA 75, 4642-4646) has been investigated by specific incorporation of 15N into the epsilon-amino groups of the lysine residues. Comparison of resonance Raman spectra of bacteriorhodopsin grown on 100%, 0%, and 50% labeled lysine demonstrates that 15N isotope effects on the Schiff base vibration can be accounted for by 15N labeling only at the Schiff base nitrogen. Our data also provide in situ confirmation of the linkage of the retinal chromophore with the epsilon-amino nitrogen of lysine.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1979 Mar 14;505(3-4):215-78 - PubMed
    1. Can J Microbiol. 1969 Oct;15(10):1157-65 - PubMed
    1. Biochemistry. 1978 Oct 31;17(22):4722-35 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4462-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Jan;76(1):227-31 - PubMed

Publication types

LinkOut - more resources