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. 1980 Nov 15;192(2):719-23.
doi: 10.1042/bj1920719.

A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine

P J Garlick, M A McNurlan, V R Preedy

A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine

P J Garlick et al. Biochem J. .

Abstract

A rapid procedure for measuring the specific radioactivity of phenylalanine in tissues was developed. This facilitates the accurate determination of rates of protein synthesis in a wide range of tissues by injection of 150 mumol of L-[4-(3)H]phenylalanine/100 g body wt. The large dose of amino acid results in a rapid rise in specific radioactivity of free phenylalanine in tissues to values close to that in plasma, followed by a slow but linear fall. This enables the rate of protein synthesis to be calculated from measurements of the specific radioactivity of free and protein-bound phenylalanine in tissues during a 10 min period after injection of radioisotope.

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