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. 1981 May 8;223(2):409-16.
doi: 10.1016/s0378-4347(00)80114-0.

Assay of alpha-difluoromethylornithine in body fluids and tissues by automatic amino-acid analysis

J Grove, J R Fozard, P S Mamont

Assay of alpha-difluoromethylornithine in body fluids and tissues by automatic amino-acid analysis

J Grove et al. J Chromatogr. .

Abstract

A procedure is described for the measurement of DL-alpha-difluoromethylornithine (DFMO), a selective irreversible inhibitor of ornithine decarboxylase, in biological specimens. The drug is separated from other amino acids with a commerical amino-acid analyser and detected by formation of its alkylthio-isoindole derivative with o-phthalaldehyde. DFMO concentrations of 0.1 nmol can be determined in a sample volume of 100 microliter. The assay has been used to determine the half-life of DFMO in serum of several species and the relationships between serum and tissue concentrations.

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