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. 1981 Jun;4(5-6):339-46.
doi: 10.1016/0165-022x(81)90074-9.

Improved methods for the fluorographic detection of weak beta-emitting radioisotopes in Agarose and acrylamide gel electrophoresis media

D E Pulleyblank, G M Booth

Improved methods for the fluorographic detection of weak beta-emitting radioisotopes in Agarose and acrylamide gel electrophoresis media

D E Pulleyblank et al. J Biochem Biophys Methods. 1981 Jun.

Abstract

The use of acetic acid as a solvent for diphenyloxazole (PPO) in fluorographic procedures has been investigated. It is demonstrated to be superior to both dimethyl sulfoxide and methanol with respect to its suitability in both agarose and acrylamide gel electrophoresis systems. In addition, a method has been developed for impregnating fragile gels such as those used for immunodiffusion with PPO in preparation for fluorography.

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