Use of enzyme-linked immunosorbent assays (ELISA) for detection of monoclonal antibodies: experience with antigens of Toxoplasma gondii

Abstract

The usefulness of enzyme-linked immunosorbent assay (ELISA) systems for detection of monoclonal antibodies to Toxoplasma gondii was studied. Seven monoclonal antibodies with specificities for membrane antigens, cytoplasmic antigens, or both membrane and cytoplasmic antigens of T. gondii were tested in a "sandwich" or in a "double sandwich" assay. Whereas both ELISA systems were sensitive and specific for detection of monoclonal antibodies to T. gondii, the double sandwich ELISA proved more sensitive than did the sandwich assay. Using different T. gondii antigen preparations in both assays, we demonstrated that the ELISA systems are specific, sensitive, rapid, and easy to perform and are therefore useful for screening and detection of monoclonal antibodies of desired isotypes and defined specificities.