Complete 3' noncoding region sequences of rabbit and human beta-globin messenger RNAs

Abstract

The 3' noncoding regions of rabbit and human beta-globin mRNAs, 95 and 134 nucleotides in length, have been sequenced. Furthermore, these and previous studies (Baralle, 1977; Proudfoot, 1976a, 1976b) have established about one half of the 589 nucleotide sequence of rabbit beta-globin mRNA, including all of the noncoding region. These sequence studies took advantage of the plus and minus gel-sequencing procedure originally described by Sanger and Coulson (1975) and recently adapted by G.G. Brownlee and E.M. Cartwright (manuscript in preparation). The human and rabbit 3' noncoding region sequences are extensively homologous, except that the human sequence has an addition of 39 nucleotides as compared with rabbit. This extra sequence may have arisen by gene duplication.