Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Oct;78(10):6376-80.
doi: 10.1073/pnas.78.10.6376.

Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring

T E Wagner, P C Hoppe, J D Jollick, D R Scholl, R L Hodinka, J B Gault

Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring

T E Wagner et al. Proc Natl Acad Sci U S A. 1981 Oct.

Abstract

We have transferred a gene coding for rabbit beta-globin into the male pronucleus of mouse zygotes by direct microinjection. Some of these zygotes developed into mature mice which contained this gene and appeared to be producing a rabbit globin. Evidence for the presence of the gene in these animals was provided by Southern blot hybridization analysis. Evidence for the expression of the rabbit gene in these transformed mice and their offspring was provided by hemoglobin isoelectric focusing analysis and specific serological reactivity between mouse anti-rabbit hemoglobin antiserum and a hemolysate from the mice that developed from the microinjected zygotes. The use of this zygote transformation may allow the introduction and expression of a broad range of genetic elements in mammals.

PubMed Disclaimer

References

    1. Cell. 1978 Jul;14(3):725-31 - PubMed
    1. J Exp Zool. 1978 Mar;203(3):371-80 - PubMed
    1. Arch Androl. 1978;1(1):31-41 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Feb;76(2):867-71 - PubMed
    1. Mol Gen Genet. 1979 Feb 26;170(2):179-85 - PubMed

Publication types