Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1981;73(1):115-20.
doi: 10.1007/BF00493137.

The use of glutaraldehyde and tannic acid to preserve reconstituted collagen for electron microscopy

K M Meek
Comparative Study

The use of glutaraldehyde and tannic acid to preserve reconstituted collagen for electron microscopy

K M Meek. Histochemistry. 1981.

Abstract

The effects of glutaraldehyde and tannic acid on the axial periodicity of collagen have measured. Both fixatives produce axial shrinkage of the collagen but whereas glutaraldehyde produces 7% shrinkage, tannic acid produces only 2% shrinkage. The technique of carbon/platinum shadowing was used to estimate the extent to which the collagen fibrils flatten down when they are dried onto grids for electron microscopy without prior embedding and sectioning. The influence of fixation was studied and it was found that minimum distortion occurred when both tannic acid and glutaraldehyde were used to preserve the protein structure.

PubMed Disclaimer

References

    1. J Mol Biol. 1979 Jun 5;130(4):511-2 - PubMed
    1. J Microsc. 1970 Feb;91(1):31-41 - PubMed
    1. Tissue Cell. 1975;7(1):211-5 - PubMed
    1. J Biophys Biochem Cytol. 1960 Jul;7:613-8 - PubMed
    1. Connect Tissue Res. 1974;2(2):151-9 - PubMed

Publication types