doi: 10.1007/BF00426002.
Transfer of nitrogen fixation genes into Pseudomonas putida isolated from Finnish tundra soil
- PMID: 6798929
- DOI: 10.1007/BF00426002
Item in Clipboard
Transfer of nitrogen fixation genes into Pseudomonas putida isolated from Finnish tundra soil
Antonie Van Leeuwenhoek.
1981 Dec.
Abstract
The plasmid pRD1 containing the nif genes from Klebsiella pneumoniae was transferred by conjugation from Escherichia coli to Pseudomonas putida isolated from the tundra soil. 6-Cyanopurine, acetylene reduction and immunological tests showed that the nif genes were not expressed in P. putida. Existence of the nif genes in P. putida transconjugants was detected by transferring them to E. coli C600, which does not fix nitrogen. Existence of the nif genes in E. coli C600 transconjugants was detected immunologically and by acetylene reduction tests.
Similar articles
-
Qualitative evidence for expression of Klebsiella pneumoniae nif in Pseudomonas putida.J Gen Microbiol. 1987 Sep;133(9):2563-6. doi: 10.1099/00221287-133-9-2563. J Gen Microbiol. 1987. PMID: 3329216
-
Transfer and expression of Klebsiella nif genes in Alcaligenes faecalis, a nitrogen-fixing bacterium associated with rice root.Plasmid. 1983 Nov;10(3):290-2. doi: 10.1016/0147-619x(83)90043-4. Plasmid. 1983. PMID: 6361825
-
Order of genes near nif in Klebsiella pneumoniae.J Bacteriol. 1979 Jun;138(3):1041-5. doi: 10.1128/jb.138.3.1041-1045.1979. J Bacteriol. 1979. PMID: 378930 Free PMC article.
-
Expression of Klebsiella his and nif genes in Serratia marcescens, Erwinia herbicola and Proteus mirabilis.Arch Microbiol. 1980 Sep;127(2):115-8. doi: 10.1007/BF00428014. Arch Microbiol. 1980. PMID: 7000024
-
Nitrogen fixation (NIF) regulatory mutants of Klebsiella: determination of the energy cost of N2 fixation in vivo.Basic Life Sci. 1977;9:95-110. doi: 10.1007/978-1-4684-0880-5_9. Basic Life Sci. 1977. PMID: 336030 Review. No abstract available.