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. 1981 Dec;47(5):405-10.
doi: 10.1007/BF00426002.

Transfer of nitrogen fixation genes into Pseudomonas putida isolated from Finnish tundra soil

H Lehtinen, P Mäntsälä

Transfer of nitrogen fixation genes into Pseudomonas putida isolated from Finnish tundra soil

H Lehtinen et al. Antonie Van Leeuwenhoek. 1981 Dec.

Abstract

The plasmid pRD1 containing the nif genes from Klebsiella pneumoniae was transferred by conjugation from Escherichia coli to Pseudomonas putida isolated from the tundra soil. 6-Cyanopurine, acetylene reduction and immunological tests showed that the nif genes were not expressed in P. putida. Existence of the nif genes in P. putida transconjugants was detected by transferring them to E. coli C600, which does not fix nitrogen. Existence of the nif genes in E. coli C600 transconjugants was detected immunologically and by acetylene reduction tests.

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