Rabbit renal cortical microsomes metabolize arachidonic acid to trihydroxyeicosatrienoic acids

Abstract

(1-14C) Eicosatetraenoic (Arachidonic) acid was incubated with microsomes from rabbit renal cortex and NADPH (1 mM) for 15 min at 37 degrees C. The products were extracted and purified by high pressure liquid chromatography. Some of the most polar metabolites were identified by gas chromatography mass spectrometry. They were 11,12,19- and 11,12,20-trihydroxy-5,8,14-eicosatrienoic acid, 14,15,19- and 14,15,20-trihydroxy-5,8,11-eicosatrienoic acid, and 11,12-dihydroxy-19-oxo-5,8,14-eicosatrienoic acid. These products were likely formed by omega-and ( omega-1)-hydroxylation of 11,12-dihydroxy-5,8,14-eicosatrienoic acid and 14,15-dihydroxy-5,8,11-eicosatrienoic acid, two recently identified metabolites of arachidonic acid in fortified rabbit kidney microsomes.