Repair of double-strand DNA breaks in Drosophila

Abstract

This study describes the repair kinetics of DNA double-strand breaks in primary and established cell cultures of Drosophila melanogaster. Double-strand breaks, induced by X-irradiation, were monitored by neutral elution. In primary cell cultures 50% of the double-strand breaks induced by 10 kR of X-rays are repaired within 45 min and 80% of the breaks are repaired within 2-3 h. Repair kinetics in established cell cultures are similar; 50% of the induced breaks are repaired within 20 min and 88% within 3 h. Mutants deficient in other types of DNA repair were also assayed for their capacity to repair double-strand breaks.