Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Sep;151(3):1222-9.
doi: 10.1128/jb.151.3.1222-1229.1982.

Insertion of bacteriophage SP beta into the citF gene of Bacillus subtilis and specialized transduction of the ilvBC-leu genes

C J Mackey, S A Zahler

Insertion of bacteriophage SP beta into the citF gene of Bacillus subtilis and specialized transduction of the ilvBC-leu genes

C J Mackey et al. J Bacteriol. 1982 Sep.

Abstract

We isolated a strain of Bacillus subtilis in which the SP beta c2 prophage is inserted into the citF (succinate dehydrogenase) gene. Defective specialized transducing particles for the ilvBC-leu genes were isolated from phage-induced lysates of this lysogen. We isolated a group of phages that differ in the amount of genetic material they carry from this region. Also, we incorporated mutant ilv and leu alleles into the genomes of several transducing phages. Our phage collection enables us to identify the cistron of new ilv and leu mutations by complementation analysis. In this process we discovered a fourth leu cistron, leuD. Characterization of the phages confirmed the published gene order: ilvB-ilvC-leuA-leuC-leuB; leuD lies to the right of leuB.

PubMed Disclaimer

References

    1. J Bacteriol. 1973 Mar;113(3):1268-72 - PubMed
    1. J Bacteriol. 1973 Nov;116(2):719-26 - PubMed
    1. J Bacteriol. 1977 Jan;129(1):556-8 - PubMed
    1. Annu Rev Genet. 1977;11:297-347 - PubMed
    1. Gene. 1979 Feb;5(2):87-91 - PubMed

Publication types