Identification of the carboxyl peptides of mouse procollagen IV and its implications for the assembly and structure of basement membrane procollagen
- PMID: 6809743
Identification of the carboxyl peptides of mouse procollagen IV and its implications for the assembly and structure of basement membrane procollagen
Abstract
Clusters of mouse PF-HR9 endoderm cells derived from teratocarcinoma PCC4-F cells were incubated with [3H]proline and [35S]methionine. The synthesis of pro alpha 1 IV and pro alpha 2 IV chains and their association into triple helically folded disulfide-linked molecules were followed. Short incubations and incubations with pactamycin showed that approximately 30,000 molecular weight collagenase-resistant peptides, which are destroyed by pepsin, form the carboxyl end of the pro alpha IV chains. While disulfide links bridge parts of individual peptides, the carboxyl peptides of the three chains of a molecule are not disulfide linked to each other. We propose that these peptides form the knob protrusion seen in electron micrographs of rotary shadowed procollagen IV molecules. The implications of these findings, especially for the relatively slow assembly of procollagen IV, are discussed.
Similar articles
-
Mouse procollagen IV. Characterization and supramolecular association.J Biol Chem. 1982 Aug 25;257(16):9796-803. J Biol Chem. 1982. PMID: 7050102
-
Biosynthesis of two subunits of type IV procollagen and of other basement membrane proteins by a human tumor cell line.Eur J Biochem. 1980 Aug;109(1):247-55. doi: 10.1111/j.1432-1033.1980.tb04790.x. Eur J Biochem. 1980. PMID: 6250836
-
Basement membrane procollagen is not converted to collagen in organ cultures of parietal yolk sac endoderm.J Biol Chem. 1976 Mar 25;251(6):1789-94. J Biol Chem. 1976. PMID: 176163
-
Biosynthesis of procollagen.Annu Rev Biochem. 1978;47:129-62. doi: 10.1146/annurev.bi.47.070178.001021. Annu Rev Biochem. 1978. PMID: 354493 Review. No abstract available.
-
The aminopropeptide of collagen.Clin Orthop Relat Res. 1981 Jul-Aug;(158):224-42. Clin Orthop Relat Res. 1981. PMID: 6168424 Review.
Cited by
-
Monoclonal antibodies against chicken type IV and V collagens: electron microscopic mapping of the epitopes after rotary shadowing.J Cell Biol. 1984 May;98(5):1637-44. doi: 10.1083/jcb.98.5.1637. J Cell Biol. 1984. PMID: 6202698 Free PMC article.
-
Basement membrane structure in situ: evidence for lateral associations in the type IV collagen network.J Cell Biol. 1987 Dec;105(6 Pt 1):2559-68. doi: 10.1083/jcb.105.6.2559. J Cell Biol. 1987. PMID: 3693393 Free PMC article.
-
Characterization of the Proprotein Convertase-Mediated Processing of Peroxidasin and Peroxidasin-like Protein.Antioxidants (Basel). 2021 Sep 30;10(10):1565. doi: 10.3390/antiox10101565. Antioxidants (Basel). 2021. PMID: 34679700 Free PMC article.
-
Targeted incorporation of collagen IV to the basement membrane: A step forward for developing extracellular protein therapies.J Biol Chem. 2025 Jul;301(7):110384. doi: 10.1016/j.jbc.2025.110384. Epub 2025 Jun 14. J Biol Chem. 2025. PMID: 40523614 Free PMC article.
-
Assembly of chick and bovine lens-capsule collagen.Biochem J. 1985 Mar 1;226(2):527-36. doi: 10.1042/bj2260527. Biochem J. 1985. PMID: 2986598 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources