Absence of novel translation products in relation to induced activity of the 93D puff in Drosophila melanogaster

Abstract

Salivary glands of Drosophila larvae were treated in vitro with benzamide or with a homogenate of heat shocked glands to specifically induce high transcriptional activity of the 93D puff. The newly synthesized 14C-amino acids labelled polypeptides in the treated and sister control glands were analysed by polyacrylamide gel electrophoresis, followed by gel autoradiography. The protein synthesis patterns in the treated glands in either case remain the same as in control glands. No novel polypeptide was seen which could be correlated with the high induced transcriptional activity of the 93D puff. This suggests that the 93D transcript/s is/are probably not translated.