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. 1982;14(4):249-55.
doi: 10.1159/000265199.

A simple and rapid method for isolation of retinal S antigen

C Dorey, J Cozette, J P Faure

A simple and rapid method for isolation of retinal S antigen

C Dorey et al. Ophthalmic Res. 1982.

Abstract

A procedure is described for the rapid isolation of S antigen from retinal tissue, by salt precipitation, gel filtration and final purification by adsorption chromatography on hydroxyapatite-agarose. The method yielded a highly purified material and allowed an excellent preservation of the antigenicity, as shown by high pathogenic activity in induction of experimental autoimmune uveoretinitis.

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