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. 1982;71(2):89-98.

[Detection, using fluorescent lectins, of specific carbohydrate chains in primordial germ cells of anuran amphibians]

[Article in French]
M Delbos, N Saidi, J D Gipouloux
  • PMID: 6814362

[Detection, using fluorescent lectins, of specific carbohydrate chains in primordial germ cells of anuran amphibians]

[Article in French]
M Delbos et al. Arch Anat Microsc Morphol Exp. 1982.

Abstract

A study of primordial germ cells (PGC) of Amphibia Anura was carried out after treatment of sections by different fluorescein isothiocyanate conjugated lectins (FITC-lectins). Specific labelling on the PGC is obtained with lectins, the activity of which is inhibited by D-galactose or N-acetyl-galactosamine. These osidic groups appear to be located more specifically on the PGC. The same labelling pattern is not obtained with lectins possessing major affinity for mannose, glucose, fucose and N-acetyl-glucosamine. Furthermore, changes in labelling pattern are observed during migration of PGC. It is suggested that D-galactose and N-acetyl-galactosamine might be related to membrane activity of PGC during migration. Ultrastructural study of the visualization of cell surface carbohydrates supplies some information on the localisation of these lectins receptors.

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