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. 1982 Jul;34(4):428-30.
doi: 10.1007/BF02411279.

Immunohistochemical demonstration of extracellular carbonic anhydrase in epiphyseal growth cartilage

T Kumpulainen, H K Väänänen

Immunohistochemical demonstration of extracellular carbonic anhydrase in epiphyseal growth cartilage

T Kumpulainen et al. Calcif Tissue Int. 1982 Jul.

Abstract

The distribution of carbonic anhydrase isoenzymes C and B in the rate epiphyseal growth cartilage was demonstrated by an immunohistochemical method. The isoenzymes were found in different locations. Isoenzyme C was in the extracellular matrix of the hypertrophic and calcifying cartilage, and no reaction was observed in the chondrocytes. In contrast, the antiserum against isoenzyme B revealed only a weak cellular staining. This supports the hypothesis that carbonic anhydrase isoenzyme C, which is the high-activity form, changes the pH in the extracellular fluid of calcifying cartilage, favoring the deposition of calcium phosphate.

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