Rapid serological diagnosis of Aspergillus fumigatus infection of the frontal sinuses and nasal chambers of the dog

Abstract

Aspergillus fumigatus precipitins in dog sera were detected by counterimmunoelectrophoresis (CIE). The procedure gave results within 90 minutes compared with 72 to 96 hours required in agar-gel double diffusion. Culture-filtrate antigens from two-week-old cultures of A fumigatus and two-day-old mycelial antigens produced the strongest reactions in CIE tests and the former antigen also revealed high titres in tests with serum from seven of 14 dogs with A fumigatus precipitins. Serum from these 14 dogs also reacted in CIE tests with a number of Penicillium species antigens.