Effect of different carbon sources on delta 5 desaturation activity of HTC cells

Abstract

The effect of different carbon sources on delta 5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in S-77 medium (fed cells). This activity was not modified if fasted cells were refed with glucose. However, the activity decreased significantly by the addition of lactalbumin hydrolysate to fasted cells. This effect can not be attributed to individual aminoacids since the addition of them to an aminoacid free medium produced no changes on delta 5 desaturation activity. The incubation of HTC cells with S-77 medium supplemented with glucose, pyruvate or glucose plus pyruvate, showed similar delta 5 desaturation activity to those cells incubated in S-77 medium alone.