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. 1982 Oct;2(10):1212-9.
doi: 10.1128/mcb.2.10.1212-1219.1982.

Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae

S J Silverman, M Rose, D Botstein, G R Fink

Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae

S J Silverman et al. Mol Cell Biol. 1982 Oct.

Abstract

The beginning of the Saccharomyces cerevisiae HIS4 gene has been fused to the structural gene for Escherichia coli beta-galactosidase. This construction, which contains HIS4 DNA from -732 to +30 relative to the translation initiation codon, has been integrated into the yeast genome at two chromosomal locations, HIS4 and URA3. At both locations, this 762-base-pair stretch of DNA is sufficient for initiating expression of beta-galactosidase activity in S. cerevisiae and confers upon this activity the regulatory response normally found for HIS4.

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