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. 1982 Sep;2(9):1033-43.
doi: 10.1128/mcb.2.9.1033-1043.1982.

Mutations affecting the structure and function of immunoglobulin M

M J Shulman, C Heusser, C Filkin, G Köhler

Mutations affecting the structure and function of immunoglobulin M

M J Shulman et al. Mol Cell Biol. 1982 Sep.

Abstract

Using a hybridoma cell line which secretes hapten-specific immunoglobulin M (IgM), we have isolated a variety of mutants which produce abnormal immunoglobulin. Immunoglobulin was tested for the size and composition of the component heavy and light chains and for variable and constant region related functional and serological activities. Some mutants secrete IgM which seems to be defective in hapten binding; others make IgM which appears not to activate complement. Many of the mutants secrete monomeric as opposed to pentameric IgM. In some cases, the defect apparently correlates with structural alterations in the mu heavy chain: partial deletion, polypeptide addition, and abnormal glycosylation have been observed. These mutant cell lines provide a means of identifying the structural basis of IgM function and of studying the biochemistry of IgM synthesis and processing.

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