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. 1982 Nov;128(11):2813-6.
doi: 10.1099/00221287-128-11-2813.

Facile in vivo transfer of mutations between the Bacillus subtilis chromosome and a plasmid harbouring homologous DNA

K F Chak, H de Lencastre, H M Liu, P J Piggot

Facile in vivo transfer of mutations between the Bacillus subtilis chromosome and a plasmid harbouring homologous DNA

K F Chak et al. J Gen Microbiol. 1982 Nov.

Abstract

Transformation of Bacillus subtilis strains carrying the point mutations spoIIA42 or spoIIA69 by a plasmid, pHM2, carrying the spoIIA+ allele gave occasional recombinants in which the plasmid had acquired the chromosomal mutation. Transformation of a spo+ strain by these derivative plasmids gave occasional recombinants in which the chromosome of the recipient had acquired the plasmid-borne spoIIA mutation. Both types of behaviour were recE+-dependent. Mutations spoIIA42 and spoIIA69 fell into a single complementation group.

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