Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981;1(1):5-11.
doi: 10.1089/hyb.1.1981.1.5.

Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies

D E Yelton, C Desaymard, M D Scharff

Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies

D E Yelton et al. Hybridoma. 1981.

Abstract

Rat monoclonal antibodies specific for mouse kappa light chains and mouse gamma heavy chains have been generated. These rat monoclonal antibodies have been biosynthetically labelled with 35S methionine. The free label was dialyzed from the medium and, without further purification, the medium containing the radioactive monoclonal antibody was used in a radioimmunoassay to screen the sera of the immunized animals and hybridomas for specific mouse antibodies of the IgG class.

PubMed Disclaimer

Publication types

LinkOut - more resources